Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer.

We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produces highly polymorphic DNA band patterns for different strains of Trypanosoma cruzi. By labeling the PCR products with digoxigenin and by chemiluminescence detection, we improved the assay sensitivity...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Clin Microbiol
Main Authors: González, N, Galindo, I, Guevara, P, Novak, E, Scorza, J V, Añez, N, Da Silveira, J F, Ramírez, J L
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 1994
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC262987/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8126172/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.32.1.153-158.1994
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