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A rapid colorimetric assay for evaluating Legionella pneumophila growth in macrophages in vitro.

A rapid colorimetric technique for in vitro quantitation of Legionella pneumophila intracellular proliferation in macrophages is described. The assay is based on the electron transport activity of metabolically active L. pneumophila. The yellow tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe...

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Bibliographic Details
Main Authors: Gebran, S J, Newton, C A, Yamamoto, Y, Klein, T W, Friedman, H
Format: Artigo
Language:Inglês
Published: 1994
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC262981/
https://ncbi.nlm.nih.gov/pubmed/8126166
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