Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.

The sensitivities of three methods of detection of Mycoplasma pneumoniae by a 16S rDNA PCR were compared by using a serial dilution of M. pneumoniae. These methods consisted of a PCR performed directly on cells after a proteinase K pretreatment (direct PCR), a PCR after purification of nucleic acids...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Tjhie, J H, van Kuppeveld, F J, Roosendaal, R, Melchers, W J, Gordijn, R, MacLaren, D M, Walboomers, J M, Meijer, C J, van den Brule, A J
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1994
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC262961/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7510308/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.32.1.11-16.1994
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