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Genotyping by multiplex polymerase chain reaction for detection of endemic hepatitis B virus transmission.

A nested polymerase chain reaction (PCR) protocol was developed for rapid genotyping of hepatitis B virus (HBV). During the first PCR round, a universal HBV primer pair was used to amplify the entire pre-S region of the HBV genome. Within the pre-S region, many nucleotide exchanges are observed. The...

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Bibliographic Details
Published in:J Clin Microbiol
Main Authors: Repp, R, Rhiel, S, Heermann, K H, Schaefer, S, Keller, C, Ndumbe, P, Lampert, F, Gerlich, W H
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1993
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC262885/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8501209/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.5.1095-1102.1993
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