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Detection of Mycoplasma pneumoniae by polymerase chain reaction and nonradioactive hybridization in microtiter plates.

In order to improve the diagnosis of a Mycoplasma pneumoniae infection, we developed a polymerase chain reaction (PCR)-based assay. The gene encoding elongation factor Tu (tuf) was selected as the target sequence. Oligonucleotides derived from variable stretches of the tuf gene were able to prime th...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Lüneberg, E, Jensen, J S, Frosch, M
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1993
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC262884/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8501208/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.5.1088-1094.1993
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