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Duplex Real-Time SYBR Green PCR Assays for Detection of 17 Species of Food- or Waterborne Pathogens in Stools

A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of s...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Fukushima, Hiroshi, Tsunomori, Yoshie, Seki, Ryotaro
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology 2003
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC262470/
https://ncbi.nlm.nih.gov/pubmed/14605150
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.11.5134-5146.2003
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