Cloning and surface expression in Escherichia coli of a structural gene encoding a surface protein of Haemophilus influenzae type b.

Recombinant DNA technology was used to clone a gene coding for a surface protein of Haemophilus influenzae type b (Hib) into Escherichia coli. Chromosomal DNA from a clinical isolate of Hib was cleaved with EcoRI and ligated into plasmid vectors containing three different translational reading frame...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Holmans, P L, Loftus, T A, Hansen, E J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC262162/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3899933/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.50.1.236-242.1985
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