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Peptide nucleic acid pre-gel hybridization: An alternative to Southern hybridization

We have found that it is possible to use labeled peptide nucleic acid (PNA)-oligomers as probes in pre-gel hybridization experiments, as an alternative for Southern hybridization. In this technique, the PNA probe is hybridized to a denatured DNA sample at low ionic strength and the mixture is loaded...

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Main Authors: Perry-O’Keefe, Heather, Yao, Xian-Wei, Coull, James M., Fuchs, Martin, Egholm, Michael
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences of the USA 1996
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC26193/
https://ncbi.nlm.nih.gov/pubmed/8962112
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