Expression, purification, and initial characterization of human alanine aminotransferase (ALT) isoenzyme 1 and 2 in High-five insect cells

Alanine aminotransferase (ALT) is a key enzyme for gluconeogenesis as well as a widely-used serum marker for liver injury. We have identified two ALT isoenzymes, ALT1 and ALT2, which are encoded by separate genes. In this study, we described the expression, purification and initial characterization...

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Detalhes bibliográficos
Main Authors: Liu, Li, Zhong, Shao, Yang, Rongze, Hu, Hong, Yu, Daozhan, Zhu, Dalong, Hua, Zichun, Shuldiner, Alan R., Goldstein, Richard, Reagan, William J., Gong, Da-Wei
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2617774/
https://ncbi.nlm.nih.gov/pubmed/18508279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.04.006
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