A general method for determining helix packing in membrane proteins in situ: Helices I and II are close to helix VII in the lactose permease of Escherichia coli
It was previously shown that coexpression of the lactose permease of Escherichia coli in two contiguous fragments leads to functional complementation. We demonstrate here that site-directed thiol crosslinking of coexpressed permease fragments can be used to determine helix proximity in situ without...
সংরক্ষণ করুন:
| প্রকাশিত: | Proc Natl Acad Sci U S A |
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| প্রধান লেখক: | , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
National Academy of Sciences
1996
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC26161/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8962080/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.25.14498 |
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