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A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large DNA viruses as bacterial artificial chromosomes
Bacterial artificial chromosomes (BACs) derived from genomes of large DNA viruses are powerful tools for functional delineation of viral genes. Current methods for cloning the genomes of large DNA viruses as BACs require prior knowledge of the viral sequences or the cloning of viral DNA fragments, a...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2009
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2615602/ https://ncbi.nlm.nih.gov/pubmed/18988631 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn890 |
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