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A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large DNA viruses as bacterial artificial chromosomes

Bacterial artificial chromosomes (BACs) derived from genomes of large DNA viruses are powerful tools for functional delineation of viral genes. Current methods for cloning the genomes of large DNA viruses as BACs require prior knowledge of the viral sequences or the cloning of viral DNA fragments, a...

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Detalhes bibliográficos
Main Authors: Zhou, Fuchun, Li, Qiuhua, Gao, Shou-Jiang
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2615602/
https://ncbi.nlm.nih.gov/pubmed/18988631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn890
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