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Forward and robust selection of the most potent and noncellular toxic siRNAs from RNAi libraries
Use of highly potent small interfering RNAs (siRNAs) can substantially reduce dose-dependent cytotoxic and off-target effects. We developed a genetic forward approach by fusing the cytosine deaminase gene with targets for the robust identification of highly potent siRNAs from RNA interference (RNAi)...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2009
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2615601/ https://ncbi.nlm.nih.gov/pubmed/19043072 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn953 |
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