Saccharomyces cerevisiae Linker Histone Hho1p Functionally Interacts with Core Histone H4 and Negatively Regulates the Establishment of Transcriptionally Silent Chromatin
Saccharomyces cerevisiae linker histone Hho1p is not essential for cell viability, and very little is known about its function in vivo. We show that deletion of HHO1 (hho1Δ) suppresses the defect in transcriptional silencing caused by a mutation in the globular domain of histone H4. hho1Δ also suppr...
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American Society for Biochemistry and Molecular Biology
2009
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2613606/ https://ncbi.nlm.nih.gov/pubmed/19017647 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M806274200 |
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pubmed-26136062010-01-09 Saccharomyces cerevisiae Linker Histone Hho1p Functionally Interacts with Core Histone H4 and Negatively Regulates the Establishment of Transcriptionally Silent Chromatin Yu, Qun Kuzmiak, Holly Zou, Yanfei Olsen, Lars Defossez, Pierre-Antoine Bi, Xin J Biol Chem Transcription, Chromatin, and Epigenetics Saccharomyces cerevisiae linker histone Hho1p is not essential for cell viability, and very little is known about its function in vivo. We show that deletion of HHO1 (hho1Δ) suppresses the defect in transcriptional silencing caused by a mutation in the globular domain of histone H4. hho1Δ also suppresses the reduction in HML silencing by the deletion of SIR1 that is involved in the establishment of silent chromatin at HML. We further show that hho1Δ suppresses changes in silent chromatin structure caused by the histone H4 mutation and sir1Δ. These results suggest that HHO1 plays a negative role in transcriptionally silent chromatin. We also provide evidence that Hho1p hinders the de novo establishment of silent chromatin but does not affect the stability of preexistent silent chromatin. Unlike canonical linker histones in higher eukaryotes that have a single conserved globular domain, Hho1p possesses two globular domains. We show that the carboxyl-terminal globular domain of Hho1p is dispensable for its function, suggesting that the mode of Hho1p action is similar to that of canonical linker histones. American Society for Biochemistry and Molecular Biology 2009-01-09 /pmc/articles/PMC2613606/ /pubmed/19017647 http://dx.doi.org/10.1074/jbc.M806274200 Text en Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc. |
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PubMed Central |
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Inglês |
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Artigo |
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Transcription, Chromatin, and Epigenetics |
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Transcription, Chromatin, and Epigenetics Yu, Qun Kuzmiak, Holly Zou, Yanfei Olsen, Lars Defossez, Pierre-Antoine Bi, Xin Saccharomyces cerevisiae Linker Histone Hho1p Functionally Interacts with Core Histone H4 and Negatively Regulates the Establishment of Transcriptionally Silent Chromatin |
| description |
Saccharomyces cerevisiae linker histone Hho1p is not essential for cell viability, and very little is known about its function in vivo. We show that deletion of HHO1 (hho1Δ) suppresses the defect in transcriptional silencing caused by a mutation in the globular domain of histone H4. hho1Δ also suppresses the reduction in HML silencing by the deletion of SIR1 that is involved in the establishment of silent chromatin at HML. We further show that hho1Δ suppresses changes in silent chromatin structure caused by the histone H4 mutation and sir1Δ. These results suggest that HHO1 plays a negative role in transcriptionally silent chromatin. We also provide evidence that Hho1p hinders the de novo establishment of silent chromatin but does not affect the stability of preexistent silent chromatin. Unlike canonical linker histones in higher eukaryotes that have a single conserved globular domain, Hho1p possesses two globular domains. We show that the carboxyl-terminal globular domain of Hho1p is dispensable for its function, suggesting that the mode of Hho1p action is similar to that of canonical linker histones. |
| author |
Yu, Qun Kuzmiak, Holly Zou, Yanfei Olsen, Lars Defossez, Pierre-Antoine Bi, Xin |
| author_facet |
Yu, Qun Kuzmiak, Holly Zou, Yanfei Olsen, Lars Defossez, Pierre-Antoine Bi, Xin |
| author_sort |
Yu, Qun |
| title |
Saccharomyces cerevisiae Linker Histone Hho1p Functionally
Interacts with Core Histone H4 and Negatively Regulates the Establishment of
Transcriptionally Silent
Chromatin |
| title_short |
Saccharomyces cerevisiae Linker Histone Hho1p Functionally
Interacts with Core Histone H4 and Negatively Regulates the Establishment of
Transcriptionally Silent
Chromatin |
| title_full |
Saccharomyces cerevisiae Linker Histone Hho1p Functionally
Interacts with Core Histone H4 and Negatively Regulates the Establishment of
Transcriptionally Silent
Chromatin |
| title_fullStr |
Saccharomyces cerevisiae Linker Histone Hho1p Functionally
Interacts with Core Histone H4 and Negatively Regulates the Establishment of
Transcriptionally Silent
Chromatin |
| title_full_unstemmed |
Saccharomyces cerevisiae Linker Histone Hho1p Functionally
Interacts with Core Histone H4 and Negatively Regulates the Establishment of
Transcriptionally Silent
Chromatin |
| title_sort |
saccharomyces cerevisiae linker histone hho1p functionally
interacts with core histone h4 and negatively regulates the establishment of
transcriptionally silent
chromatin |
| publisher |
American Society for Biochemistry and Molecular Biology |
| publishDate |
2009 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC2613606/ https://ncbi.nlm.nih.gov/pubmed/19017647 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M806274200 |
| _version_ |
1751570969963528192 |