Cloning of extracellular DNase and construction of a DNase-negative strain of Vibrio cholerae.
The structural gene xds for extracellular DNase of Vibrio cholerae was cloned and inactivated by insertion of the transposon Tn5. The inactivated gene was introduced into the chromosome of V. cholerae by recombination to construct an extracellular DNase-negative strain. Tn5-mediated transposon-facil...
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| Udgivet i: | Infect Immun |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC261359/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2982738/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.47.3.691-696.1985 |
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