Specific Detection of Viable Legionella Cells by Combined Use of Photoactivated Ethidium Monoazide and PCR/Real-Time PCR

Legionella organisms are prevalent in manmade water systems and cause legionellosis in humans. A rapid detection method for viable Legionella cells combining ethidium monoazide (EMA) and PCR/real-time PCR was assessed. EMA could specifically intercalate and cleave the genomic DNA of heat- and chlori...

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Bibliographic Details
Main Authors: Chang, Bin, Sugiyama, Kanji, Taguri, Toshitsugu, Amemura-Maekawa, Junko, Kura, Fumiaki, Watanabe, Haruo
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2612233/
https://ncbi.nlm.nih.gov/pubmed/18978073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00604-08
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