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Generation of Large Chromosomal Deletions in Koji Molds Aspergillus oryzae and Aspergillus sojae via a Loop-Out Recombination

We established a technique for efficiently generating large chromosomal deletions in the koji molds Aspergillus oryzae and A. sojae by using a ku70-deficient strain and a bidirectional marker. The approach allowed deletion of 200-kb and 100-kb sections of A. oryzae and A. sojae, respectively. The de...

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Detalhes bibliográficos
Main Authors: Takahashi, Tadashi, Jin, Feng Jie, Sunagawa, Misao, Machida, Masayuki, Koyama, Yasuji
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2607183/
https://ncbi.nlm.nih.gov/pubmed/18952883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00692-08
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