Enhanced Transformation Efficiency of Recalcitrant Bacillus cereus and Bacillus weihenstephanensis Isolates upon In Vitro Methylation of Plasmid DNA

Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation...

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Autors principals: Groot, Masja Nierop, Nieboer, Frank, Abee, Tjakko
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2008
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2607179/
https://ncbi.nlm.nih.gov/pubmed/18952874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01932-08
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