Sandwich Hybridization Assay for Sensitive Detection of Dynamic Changes in mRNA Transcript Levels in Crude Escherichia coli Cell Extracts in Response to Copper Ions

Transcript quantification techniques usually rely on purified mRNAs. We report here a solution-based sandwich hybridization assay for the quantification of mRNAs from Escherichia coli without the need of prior RNA isolation. This assay makes use of four DNA oligonucleotide probes adjacently hybridiz...

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Autori principali: Thieme, Daniel, Neubauer, Peter, Nies, Dietrich H., Grass, Gregor
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 2008
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2607146/
https://ncbi.nlm.nih.gov/pubmed/18952865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01370-08
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