Maintenance of biological activity of pertussis toxin radioiodinated while bound to fetuin-agarose.
We developed a method to produce radioiodinated pertussis toxin (PT) which was active in the goose erythrocyte agglutination and CHO cell assay systems. The procedure used fetuin coupled to agarose to prevent inactivation of the toxin during the iodination reaction. Analysis of the labeled PT by aff...
Uloženo v:
| Vydáno v: | Infect Immun |
|---|---|
| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1987
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260504/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2437034/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.5.1294-1299.1987 |
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