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Cloning of the filamentous hemagglutinin of Bordetella pertussis and its expression in Escherichia coli.

Bordetella pertussis UT25 DNA was cloned into the kanamycin resistance gene of cosmid pCP13 to construct a genomic library in Escherichia coli LE392. One clone containing plasmid pDB441 expressed the filamentous hemagglutinin (FHA) as identified by protein immunoblots with the use of rabbit anti-B....

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Infect Immun
Prif Awduron: Brown, D R, Parker, C D
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology (ASM) 1987
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260294/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2878885/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.1.154-161.1987
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