Cloning of the filamentous hemagglutinin of Bordetella pertussis and its expression in Escherichia coli.
Bordetella pertussis UT25 DNA was cloned into the kanamycin resistance gene of cosmid pCP13 to construct a genomic library in Escherichia coli LE392. One clone containing plasmid pDB441 expressed the filamentous hemagglutinin (FHA) as identified by protein immunoblots with the use of rabbit anti-B....
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| Veröffentlicht in: | Infect Immun |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1987
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260294/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2878885/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.1.154-161.1987 |
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