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Expression of Streptococcus sanguis antigens in Escherichia coli: cloning of a structural gene for adhesion fimbriae.

Chromosomal DNA from Streptococcus sanguis FW213 was partially digested with EcoRI and ligated into the positive-selection cloning vector pOP203(A2+). The ligation mixture was used to transform Escherichia coli K-12, and 4,500 transformants were examined. The tetracycline-resistant colonies had inse...

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Hlavní autoři: Fives-Taylor, P M, Macrina, F L, Pritchard, T J, Peene, S S
Médium: Artigo
Jazyk:Inglês
Vydáno: 1987
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC260289/
https://ncbi.nlm.nih.gov/pubmed/2878882
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