One-step isolation of plasma membrane proteins using magnetic beads with immobilized concanavalin A

We have developed a simple method for isolating and purifying plasma membrane proteins from various cell types. This one-step affinity-chromatography method uses the property of the lectin concanavalin A (ConA) and the technique of magnetic-bead separation to obtain highly purified plasma membrane p...

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Autors principals: Lee, Yu-Chen, Block, Gregory, Chen, Huiwen, Folch-Puy, Emma, Foronjy, Robert, Jalili, Roxana, Jendresen, Christian Bille, Kimura, Masashi, Kraft, Edward, Lindemose, Søren, Lu, Jin, McLain, Teri, Nutt, Leta, Ramon-Garcia, Santiago, Smith, Joseph, Spivak, Aaron, Wang, Michael L., Zanic, Marija, Lin, Sue-Hwa
Format: Artigo
Idioma:Inglês
Publicat: 2008
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2600885/
https://ncbi.nlm.nih.gov/pubmed/18765283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.08.003
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