Quantitation of monomeric and oligomeric forms of membrane-bound staphylococcal alpha-toxin by enzyme-linked immunosorbent assay with a neutralizing monoclonal antibody.

A murine monoclonal antibody generated against staphylococcal alpha-toxin was shown to react only with the monomeric (native), 3S form of the toxin. A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) constructed with this antibody permitted detection of 0.25 to 0.5 ng of native toxin per...

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Hlavní autoři: Hugo, F, Sinner, A, Reichwein, J, Bhakdi, S
Médium: Artigo
Jazyk:Inglês
Vydáno: 1987
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC260009/
https://ncbi.nlm.nih.gov/pubmed/3679538
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