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Quantitation of monomeric and oligomeric forms of membrane-bound staphylococcal alpha-toxin by enzyme-linked immunosorbent assay with a neutralizing monoclonal antibody.

A murine monoclonal antibody generated against staphylococcal alpha-toxin was shown to react only with the monomeric (native), 3S form of the toxin. A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) constructed with this antibody permitted detection of 0.25 to 0.5 ng of native toxin per...

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Detalles Bibliográficos
Publicado en:Infect Immun
Principais autores: Hugo, F, Sinner, A, Reichwein, J, Bhakdi, S
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1987
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260009/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3679538/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.12.2933-2939.1987
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