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Combining Fluorescence Lifetime and Polarization Microscopy to Discriminate Phase Separated Domains in Giant Unilamellar Vesicles

Using fluorescence lifetime microscopy we study the structure of lipid domains in giant unilamellar vesicles made from sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and cholesterol. Lifetimes and orientation of a derivative of the fluorescent probe DPH embedded in the membrane were measur...

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Détails bibliographiques
Auteurs principaux: Haluska, Christopher K., Schröder, André P., Didier, Pascal, Heissler, Denis, Duportail, Guy, Mély, Yves, Marques, Carlos M.
Format: Artigo
Langue:Inglês
Publié: The Biophysical Society 2008
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2599849/
https://ncbi.nlm.nih.gov/pubmed/18790852
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.131490
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