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Comprehensive in silico mutagenesis highlights functionally important residues in proteins

Motivation: Mutating residues into alanine (alanine scanning) is one of the fastest experimental means of probing hypotheses about protein function. Alanine scans can reveal functional hot spots, i.e. residues that alter function upon mutation. In vitro mutagenesis is cumbersome and costly: probing...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Bromberg, Yana, Rost, Burkhard
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2597370/
https://ncbi.nlm.nih.gov/pubmed/18689826
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/bioinformatics/btn268
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