Isolation of Shiga toxin-resistant Vero cells and their use for easy identification of the toxin.
Shiga toxin-resistant Vero cells were isolated by treatment of the cells with nitrosoguanidine. These mutant cells were not affected by Shiga toxin at more than 1 microgram/ml, although the parent Vero cells were sensitive to 25 pg of the toxin per ml. Immunofluorescence studies showed that all the...
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| Udgivet i: | Infect Immun |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1988
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC259593/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3045003/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.56.9.2491-2494.1988 |
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