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A Strategy to Setup Codominant Microsatellite Analysis for High-Resolution-Melting-Curve-Analysis (HRM)

BACKGROUND: High resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA. The measurement is immediately following PCR in a one-step, closed-tube method. The shape of the melti...

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Bibliographic Details
Main Authors: Mader, Eduard, Lukas, Brigitte, Novak, Johannes
Format: Artigo
Language:Inglês
Published: BioMed Central 2008
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2588637/
https://ncbi.nlm.nih.gov/pubmed/18980665
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2156-9-69
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