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Expression in Escherichia coli of the 37-kilodalton endoflagellar sheath protein of Treponema pallidum by use of the polymerase chain reaction and a T7 expression system.

We previously reported the complete primary structure of the 37-kilodalton endoflagellar sheath protein (FlaA) of Treponema pallidum. However, we were unable to determine the nucleotide sequence of flaA upstream of amino acid 10. The desired nucleotide sequence was obtained by use of a strategy base...

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Dettagli Bibliografici
Pubblicato in:Infect Immun
Autori principali: Isaacs, R D, Radolf, J D
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1990
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC258771/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2194955/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.58.7.2025-2034.1990
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