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WHEAT GERM CELL-FREE TRANSLATION, PURIFICATION, AND ASSEMBLY OF A FUNCTIONAL HUMAN STEAROYL-COA DESATURASE COMPLEX

A wheat germ cell-free extract was used to perform in vitro translation of human stearoyl-CoA desaturase in the presence of unilamelar liposomes, and near complete transfer of the expressed integral membrane protein into the liposome was observed. Moreover, co-translation of the desaturase along wit...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Goren, Michael A., Fox, Brian G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2586813/
https://ncbi.nlm.nih.gov/pubmed/18765284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.08.002
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