Electron-Electron Distances in Spin-Labeled Low-Spin Metmyoglobin Variants by Relaxation Enhancement

Thirteen single-cysteine variants of myoglobin were prepared by overexpression of apoprotein, spin labeling, and reconstitution with hemin. This procedure resulted in a protein with fewer hemichrome impurities than was obtained by an overexpression of holo-protein followed by spin labeling. Coordina...

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Bibliographic Details
Main Authors: Ulyanov, Dmitriy, Bowler, Bruce E., Eaton, Gareth R., Eaton, Sandra S.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2008
Subjects:
Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2586584/
https://ncbi.nlm.nih.gov/pubmed/18775958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.141887
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