Purification and characterization of an Escherichia coli Shiga-like toxin II variant.
A Shiga-like toxin II variant was purified to homogeneity from Escherichia coli TB1(pCG6), which contained the toxin genes cloned in multicopy plasmid pUC18. The purification scheme involved polymyxin B extraction of the toxin from bacterial cells, followed by differential (NH4)2SO4 precipitation, a...
Guardat en:
| Publicat a: | Infect Immun |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1990
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC258614/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2182539/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.58.5.1232-1239.1990 |
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