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Construction and characterization in vivo of Bordetella pertussis aroA mutants.

A DNA fragment encoding a kanamycin resistance determinant was used to insertionally inactivate the cloned aroA gene of Bordetella pertussis in Escherichia coli K-12, and a conjugative shuttle vector system based on the suicide vector pRTP1 was used to deliver the mutations from E. coli back into B....

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Infect Immun
Hauptverfasser: Roberts, M, Maskell, D, Novotny, P, Dougan, G
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1990
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC258526/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2407655/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.58.3.732-739.1990
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