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The RNA-Binding Domain of Bacteriophage P22 N Protein Is Highly Mutable, and a Single Mutation Relaxes Specificity toward λ

Antitermination in bacteriophage P22, a lambdoid phage, uses the arginine-rich domain of the N protein to recognize boxB RNAs in the nut site of two regulated transcripts. Using an antitermination reporter system, we screened libraries in which each nonconserved residue in the RNA-binding domain of...

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Detalhes bibliográficos
Main Authors: Cocozaki, Alexis I., Ghattas, Ingrid R., Smith, Colin A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583623/
https://ncbi.nlm.nih.gov/pubmed/18820025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00997-08
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