Brucella abortus Strain RB51 Leucine Auxotroph as an Environmentally Safe Vaccine for Plasmid Maintenance and Antigen Overexpression

To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abo...

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Autors principals: Rajasekaran, Parthiban, Seleem, Mohamed N., Contreras, Andrea, Purwantini, Endang, Schurig, Gerhardt G., Sriranganathan, Nammalwar, Boyle, Stephen M.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2008
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583484/
https://ncbi.nlm.nih.gov/pubmed/18836016
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01511-08
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