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New Strategy for Isolating Novel Nematicidal Crystal Protein Genes from Bacillus thuringiensis Strain YBT-1518

We have developed a strategy for isolating cry genes from Bacillus thuringiensis. The key steps are the construction of a DNA library in an acrystalliferous B. thuringiensis host strain and screening for the formation of crystal through optical microscopy observation and sodium dodecyl sulfate-polya...

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Detalhes bibliográficos
Main Authors: Guo, Suxia, Liu, Mei, Peng, Donghai, Ji, Sisi, Wang, Pengxia, Yu, Ziniu, Sun, Ming
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583473/
https://ncbi.nlm.nih.gov/pubmed/18820056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01346-08
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