Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface

Here, we describe a multi-parametric study of DNA hybridization to probes with 20–70% G + C content. Probes were designed towards 71 different sites/mutations in the phenylalanine hydroxylase gene. Seven probe lengths, three spacer lengths and six stringencies were systematically varied. The three s...

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Bibliographic Details
Main Authors:	Poulsen, Lena, Søe, Martin Jensen, Snakenborg, Detlef, Møller, Lisbeth Birk, Dufva, Martin
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 2008
Subjects:	Methods Online
Online Access:	https://ncbi.nlm.nih.gov/pmc/articles/PMC2582620/ https://ncbi.nlm.nih.gov/pubmed/18805905 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn600
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Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface

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