Long, Processive Enzymatic Dna Synthesis Using 100% Dye-Labeled Terminal Phosphate-Linked Nucleotides

We demonstrate the efficient synthesis of DNA with complete replacement of the four deoxyribonucleoside triphosphate (dNTP) substrates with nucleotides carrying fluorescent labels. A different, spectrally separable fluorescent dye suitable for single molecule fluorescence detection was conjugated to...

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Main Authors: Korlach, Jonas, Bibillo, Arek, Wegener, Jeffrey, Peluso, Paul, Pham, Thang T., Park, Insil, Clark, Sonya, Otto, Geoff A., Turner, Stephen W.
格式: Artigo
語言:Inglês
出版: Taylor & Francis 2008
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Original Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2582155/
https://ncbi.nlm.nih.gov/pubmed/18711669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15257770802260741
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