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Evaluation of real-time RT-PCR for the quantification of FCoV shedding in the faeces of domestic cats

Faecal samples were taken from cats living in multi-cat households with endemic feline coronavirus (FCoV) infection. Total RNA was extracted from faecal suspensions and FCoV RNA was quantified using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay. The real-time RT-PCR thre...

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Main Authors: Dye, Charlotte, Helps, Christopher R., Siddell, Stuart G.
格式: Artigo
語言:Inglês
出版: ESFM and AAFP. Published by Elsevier Ltd. 2008
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2582154/
https://ncbi.nlm.nih.gov/pubmed/18243744
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jfms.2007.10.010
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