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Expression, purification, crystallization and preliminary X-ray diffraction analysis of Sagittaria sagittifolia arrowhead protease inhibitor API-A in complex with bovine trypsin

Protease inhibitors play key roles in physiological processes. Arrowhead protease inhibitor A (API-A), a member of the serine protease inhibitor family, can inhibit two trypsin molecules simultaneously. In the present work, API-A from Sagittaria sagittifolia has been cloned, expressed, purified and...

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Detalhes bibliográficos
Main Authors: Jiang, Chunhui, Bao, Rui, Chen, Yuxing
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2581705/
https://ncbi.nlm.nih.gov/pubmed/18997342
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108032454
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