A green fluorescent protein-reporter mammalian two-hybrid system with extrachromosomal maintenance of a prey expression plasmid: Application to interaction screening
An improved mammalian two-hybrid system designed for interaction trap screening is described in this paper. CV-1/EBNA-1 monkey kidney epithelial cells expressing Epstein–Barr virus nuclear antigen 1 (EBNA-1) were stably transfected with a reporter plasmid for GAL4-dependent expression of the green f...
محفوظ في:
| الحاوية / القاعدة: | Proc Natl Acad Sci U S A |
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| المؤلفون الرئيسيون: | , , , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
National Academy of Sciences
2000
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC25809/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10805780/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.10.5220 |
| الوسوم: |
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