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Detection and selective dissociation of intact ribosomes in a mass spectrometer

Intact Escherichia coli ribosomes have been projected into the gas phase of a mass spectrometer by means of nanoflow electrospray techniques. Species with mass/charge ratios in excess of 20,000 were detected at the level of individual ions by using time-of-flight analysis. Once in the gas phase the...

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Published in:Proc Natl Acad Sci U S A
Main Authors: Rostom, Adam A., Fucini, Paola, Benjamin, Dennis R., Juenemann, Ralf, Nierhaus, Knud H., Hartl, F. Ulrich, Dobson, Christopher M., Robinson, Carol V.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2000
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC25803/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10805779/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.10.5185
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