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Purification and characterization of an extracellular 29-kilodalton phospholipase C from Listeria monocytogenes.

We purified and characterized an extracellular phospholipase produced by Listeria monocytogenes. This enzyme was separated as a homogeneous protein of 29 kDa by chromatography on DEAE-52 cellulose and Bio-Gel P100 columns. It is a zinc-dependent phospholipase C (PLC) that is mainly active at pH 6 to...

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Detalhes bibliográficos
Main Authors: Geoffroy, C, Raveneau, J, Beretti, J L, Lecroisey, A, Vazquez-Boland, J A, Alouf, J E, Berche, P
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC258022/
https://ncbi.nlm.nih.gov/pubmed/1904842
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