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Expression, purification and characterization of the secreted luciferase of the copepod Metridia longa from Sf9 insect cells
Metridia luciferase is a secreted luciferase from a marine copepod and uses coelenterazine as a substrate to produce a blue bioluminescence (λ(max) = 480 nm). This luciferase has been successfully applied as a bioluminescent reporter in mammalian cells. The main advantage of secreted luciferase as a...
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| Autores principales: | , , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2577724/ https://ncbi.nlm.nih.gov/pubmed/18595733 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.05.013 |
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