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Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA

BACKGROUND: cDNA-AFLP is a transcriptomics technique which does not require prior sequence information and can therefore be used as a gene discovery tool. The method is based on selective amplification of cDNA fragments generated by restriction endonucleases, electrophoretic separation of the produc...

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Detalhes bibliográficos
Main Authors: Weiberg, Arne, Pöhler, Dirk, Morgenstern, Burkhard, Karlovsky, Petr
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2577664/
https://ncbi.nlm.nih.gov/pubmed/18851732
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-9-480
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