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Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA
BACKGROUND: cDNA-AFLP is a transcriptomics technique which does not require prior sequence information and can therefore be used as a gene discovery tool. The method is based on selective amplification of cDNA fragments generated by restriction endonucleases, electrophoretic separation of the produc...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
BioMed Central
2008
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2577664/ https://ncbi.nlm.nih.gov/pubmed/18851732 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-9-480 |
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