Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles

Here we demonstrate rapid production of solubilized and functional membrane protein by simultaneous cell-free expression of an apolipoprotein and a membrane protein in the presence of lipids, leading to the self-assembly of membrane protein-containing nanolipoprotein particles (NLPs). NLPs have show...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Cappuccio, Jenny A., Blanchette, Craig D., Sulchek, Todd A., Arroyo, Erin S., Kralj, Joel M., Hinz, Angela K., Kuhn, Edward A., Chromy, Brett A., Segelke, Brent W., Rothschild, Kenneth J., Fletcher, Julia E., Katzen, Federico, Peterson, Todd C., Kudlicki, Wieslaw A., Bench, Graham, Hoeprich, Paul D., Coleman, Matthew A.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Biochemistry and Molecular Biology 2008
Gaiak:
Research
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2577204/
https://ncbi.nlm.nih.gov/pubmed/18603642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M800191-MCP200
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