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Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay

This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

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Detalles Bibliográficos
Main Authors: Guthrie, J. L., Seah, C., Brown, S., Tang, P., Jamieson, F., Drews, S. J.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2008
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2576621/
https://ncbi.nlm.nih.gov/pubmed/18784312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01551-08
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