Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes

The identification of interaction partners in protein complexes is a major goal in cell biology. Here we present a reliable affinity purification strategy to identify specific interactors that combines quantitative SILAC-based mass spectrometry with characterization of common contaminants binding to...

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Autors principals: Trinkle-Mulcahy, Laura, Boulon, Séverine, Lam, Yun Wah, Urcia, Roby, Boisvert, François-Michel, Vandermoere, Franck, Morrice, Nick A., Swift, Sam, Rothbauer, Ulrich, Leonhardt, Heinrich, Lamond, Angus
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 2008
Matèries:
Research Articles
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2568020/
https://ncbi.nlm.nih.gov/pubmed/18936248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.200805092
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