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Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes
The identification of interaction partners in protein complexes is a major goal in cell biology. Here we present a reliable affinity purification strategy to identify specific interactors that combines quantitative SILAC-based mass spectrometry with characterization of common contaminants binding to...
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| Główni autorzy: | , , , , , , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
The Rockefeller University Press
2008
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2568020/ https://ncbi.nlm.nih.gov/pubmed/18936248 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.200805092 |
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