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Effect of leucine-to-methionine substitutions on the diffraction quality of histone chaperone SET/TAF-Iβ/INHAT crystals

One of the most frequent problems in crystallization is poor quality of the crystals. In order to overcome this obstacle several methods have been utilized, including amino-acid substitutions of the target protein. Here, an example is presented of crystal-quality improvement by leucine-to-methionine...

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Autori principali: Senda, Miki, Muto, Shinsuke, Horikoshi, Masami, Senda, Toshiya
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2564889/
https://ncbi.nlm.nih.gov/pubmed/18931446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108028704
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