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Formation of Template-Switching Artifacts by Linear Amplification

Linear amplification is a method of synthesizing single-stranded DNA from either a single-stranded DNA or one strand of a double-stranded DNA. In this protocol, molecules of a single primer DNA are extended by multiple rounds of DNA synthesis at high temperature using thermostable DNA polymerases. A...

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Detalles Bibliográficos
Main Authors: Chakravarti, Dhrubajyoti, Mailander, Paula C.
Formato: Artigo
Idioma:Inglês
Publicado: The Association of Biomolecular Resource Facilities 2008
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2563923/
https://ncbi.nlm.nih.gov/pubmed/19137105
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