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In situ hybridization: general infectivity assay for retroviruses.

We have devised a general infectivity assay for retroviruses. A virus-specific [32P]DNA probe is hybridized in situ to a monolayer culture, and foci of infected cells in the monolayer are detected by exposure of the hybridized culture to X-ray films. The method is quantitative, in that it gives the...

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Detalhes bibliográficos
Main Authors: Rein, A, Rice, N, Simek, S, Cohen, M, Mural, R J
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC256217/
https://ncbi.nlm.nih.gov/pubmed/6292448
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